VEGF 121/VEGFA (AA 207-327) (Active) Protein

Produktnummer ABIN667096

Recombinant -Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für VEGF 121/VEGFA (AA 207-327) (Active) Protein (ABIN667096)

Target: VEGF 121/VEGFA

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Activity Assay (AcA), SDS-PAGE (SDS)

Reinheit: > 95 %

Produktdetails

Proteineigenschaft: AA 207-327

Sequenz: MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQEKCDKP RR

Produktmerkmale: VEGF121, 207-327aa, Human, Recombinant, E.coli

Aufreinigung: > 95% by SDS-PAGE

Endotoxin-Niveau: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED50 range ≤4ng/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: VEGF 121/VEGFA

Hintergrund: Vascular endothelial growth factor (VEGF) is homodimeric, heparin-binding glycoprotein involved in both angiogenesis and vasculogenesis. VEGF is expressed as multiple alternately spliced isoforms of VEGF121, 165, 189 and 206. VEGF binds to the receptor tyrosine kinases VEGF R1 (Flt-1) and VEGF R2 (KDR/Flk-1) to activate signal transduction and regulate both physiological and pathological angiogenesis. Recombinant VEGF121 protein was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Molekulargewicht: 14.2 kDa (122aa) confirmed by MALDI-TOF

NCBI Accession: NP_001020541